IL-31 ELISA Kit

Produktnummer ABIN5026938

Human IL-31 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human IL-31.

Kurzübersicht für IL-31 ELISA Kit (ABIN5026938)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-31 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-31 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 4.69 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-humanIL-31 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-31 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-31 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-31 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Andere Bezeichnung: IL-31 (Interleukin-31)